avidin biotin complex kit (Vector Laboratories)
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Vector Laboratories
avidin biotin complex kit
Avidin Biotin Complex Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 12774 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vectastain+avidin+biotin+complex+abc+kit/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pmc13122667-89-14-17
Average 99 stars, based on 12774 article reviews
Avidin Biotin Complex Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 12774 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vectastain+avidin+biotin+complex+abc+kit/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pmc13122667-89-14-17
Average 99 stars, based on 12774 article reviews
avidin biotin complex kit - by Bioz Stars,
2026-09
99/100 stars
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Incubation:Article Title: The distribution of gonadotropin-inhibitory hormone (GnIH) and its receptor in zebra finch (Taeniopygia guttata) spinal cord. Article Snippet: Gonadotropin-inhibitory hormone (GnIH) is a neuropeptide that typically acts in the hypothalamic-pituitary–gonadal (HPG) axis to regulate reproductive activity and sociosexual behaviors.. GnIH is synthesized in the brain and in the gonads, where it can act via its cognate receptor.. However, immunohistological evidence in songbirds also shows GnIH projections towards the brainstem. Article Title: Alzheimer’s Disease-Related Mutations in APP Influence Colorectal Tumor Formation in a Sex-Dependent Manner Article Snippet: Subsequently, the sections were incubated with primary antibody: APP (1:500, ab32136, Abcam, Cambridge, MA, USA), TACE (1:1000, PA5-27395, Invitrogen, ThermoFisher Scientific, Waltham, MA, USA), PS1 (1:500, PA5-119872, Invitrogen, ThermoFisher Scientific, Waltham, MA, USA), BACE (1:1000, #5606, Cell Signaling Technology, Inc., Danvers, MA, USA), CD68 (1:1000, MCA1957, Bio-Rad Laboratories, Inc., CA, USA), CD86 (1:675, #19589, Cell Signaling Technology, Inc., Danvers, MA, USA), CD163 (1:500, ab182422, Abcam, Cambridge, MA, USA), PGP9.5 (1:1000, ab108986, Abcam, Cambridge, MA, USA), ChAT (1:2000, ab178850, Abcam, Cambridge, MA, USA), and TH (1:3000, ab137869, Abcam, Cambridge, MA, USA) at 4°C overnight. .. On the following day, tissues were incubated using biotinylated secondary antibody for 2 h. To visualize the antibody binding, a Article Title: Dietary Supplementation of Edible Mushroom Phallus atrovolvatus Aqueous Extract Attenuates Brain Changes in the App NL−G−F Mouse Model of Alzheimer’s Disease Article Snippet: After antigen retrieval, the brain sections were subsequently incubated in 0.3% H 2 O 2 for 5 min, then blocked in PBS containing 5% normal goat serum (Equitech-Bio, Kerrville, TX, USA), 0.5% bovine serum albumin (Equitech-Bio, Kerrville, TX, USA), 0.1% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA), and 0.02% sodium azide for at least 1 h. Afterward, the sections were incubated in the primary antibody at 4 °C overnight, washed, and incubated in a biotinylated secondary antibody (1:2000) for 2 h at room temperature. .. Next, the sections were further incubated using a Article Title: Dietary Supplementation of Edible Mushroom Phallus atrovolvatus Aqueous Extract Attenuates Brain Changes in the App NL-G-F Mouse Model of Alzheimer's Disease. Article Snippet: After antigen retrieval, the brain sections were subsequently incubated in 0.3% H2O2 for 5 min, then blocked in PBS containing 5% normal goat serum (Equitech-Bio, Kerrville, TX, USA), 0.5% bovine serum albumin (Equitech-Bio, Kerrville, TX, USA), 0.1% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA), and 0.02% sodium azide for at least 1 h. Afterward, the sections were incubated in the primary antibody at 4 ◦C overnight, washed, and incubated in a biotinylated secondary antibody (1:2000) for 2 h at room temperature. .. Next, the sections were further incubated using a Avidin-Biotin Assay:Article Title: The distribution of gonadotropin-inhibitory hormone (GnIH) and its receptor in zebra finch (Taeniopygia guttata) spinal cord. Article Snippet: Gonadotropin-inhibitory hormone (GnIH) is a neuropeptide that typically acts in the hypothalamic-pituitary–gonadal (HPG) axis to regulate reproductive activity and sociosexual behaviors.. GnIH is synthesized in the brain and in the gonads, where it can act via its cognate receptor.. However, immunohistological evidence in songbirds also shows GnIH projections towards the brainstem. Article Title: Alzheimer’s Disease-Related Mutations in APP Influence Colorectal Tumor Formation in a Sex-Dependent Manner Article Snippet: Subsequently, the sections were incubated with primary antibody: APP (1:500, ab32136, Abcam, Cambridge, MA, USA), TACE (1:1000, PA5-27395, Invitrogen, ThermoFisher Scientific, Waltham, MA, USA), PS1 (1:500, PA5-119872, Invitrogen, ThermoFisher Scientific, Waltham, MA, USA), BACE (1:1000, #5606, Cell Signaling Technology, Inc., Danvers, MA, USA), CD68 (1:1000, MCA1957, Bio-Rad Laboratories, Inc., CA, USA), CD86 (1:675, #19589, Cell Signaling Technology, Inc., Danvers, MA, USA), CD163 (1:500, ab182422, Abcam, Cambridge, MA, USA), PGP9.5 (1:1000, ab108986, Abcam, Cambridge, MA, USA), ChAT (1:2000, ab178850, Abcam, Cambridge, MA, USA), and TH (1:3000, ab137869, Abcam, Cambridge, MA, USA) at 4°C overnight. .. On the following day, tissues were incubated using biotinylated secondary antibody for 2 h. To visualize the antibody binding, a Article Title: Dietary Supplementation of Edible Mushroom Phallus atrovolvatus Aqueous Extract Attenuates Brain Changes in the App NL−G−F Mouse Model of Alzheimer’s Disease Article Snippet: After antigen retrieval, the brain sections were subsequently incubated in 0.3% H 2 O 2 for 5 min, then blocked in PBS containing 5% normal goat serum (Equitech-Bio, Kerrville, TX, USA), 0.5% bovine serum albumin (Equitech-Bio, Kerrville, TX, USA), 0.1% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA), and 0.02% sodium azide for at least 1 h. Afterward, the sections were incubated in the primary antibody at 4 °C overnight, washed, and incubated in a biotinylated secondary antibody (1:2000) for 2 h at room temperature. .. Next, the sections were further incubated using a Article Title: Dietary Supplementation of Edible Mushroom Phallus atrovolvatus Aqueous Extract Attenuates Brain Changes in the App NL-G-F Mouse Model of Alzheimer's Disease. Article Snippet: After antigen retrieval, the brain sections were subsequently incubated in 0.3% H2O2 for 5 min, then blocked in PBS containing 5% normal goat serum (Equitech-Bio, Kerrville, TX, USA), 0.5% bovine serum albumin (Equitech-Bio, Kerrville, TX, USA), 0.1% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA), and 0.02% sodium azide for at least 1 h. Afterward, the sections were incubated in the primary antibody at 4 ◦C overnight, washed, and incubated in a biotinylated secondary antibody (1:2000) for 2 h at room temperature. .. Next, the sections were further incubated using a Article Title: Alzheimer’s Disease-Related Mutations in APP Influence Colorectal Tumor Formation in a Sex-Dependent Manner Article Snippet: .. Signal detection was performed using the Article Title: Acute LPS exposure enhances susceptibility to peripheral prion infection. Article Snippet: Macrophages were detected in the spleen using F4/80 mAb (clone CI: A3-1; Bio-Rad Laboratories; 1/100 dilution). .. Following addition of biotinylated secondary antibodies, immunostaining was revealed using the Article Title: Melatonin alleviates neuroinflammation in ischemic stroke by regulating cGAS-mediated microglial pyroptosis signaling Article Snippet: The slides were incubated overnight at 4°C with the following primary antibodies: goat anti-IL-1β (1:200; R and D Systems, Minneapolis, MN, USA; Cat# MAB401, RRID: AB_2124620); mouse anti-caspase-1 (1:200, BioLegend, San Diego, CA, USA, Cat# 645102, RRID: AB_2068900); and mouse anti-GSDMD (1:100, Santa Cruz Biotechnology, Dallas, TX, USA, Cat# sc-393581, RRID: AB_2819179). .. Using biotinylated secondary antibodies, the Binding Assay:Article Title: Alzheimer’s Disease-Related Mutations in APP Influence Colorectal Tumor Formation in a Sex-Dependent Manner Article Snippet: Subsequently, the sections were incubated with primary antibody: APP (1:500, ab32136, Abcam, Cambridge, MA, USA), TACE (1:1000, PA5-27395, Invitrogen, ThermoFisher Scientific, Waltham, MA, USA), PS1 (1:500, PA5-119872, Invitrogen, ThermoFisher Scientific, Waltham, MA, USA), BACE (1:1000, #5606, Cell Signaling Technology, Inc., Danvers, MA, USA), CD68 (1:1000, MCA1957, Bio-Rad Laboratories, Inc., CA, USA), CD86 (1:675, #19589, Cell Signaling Technology, Inc., Danvers, MA, USA), CD163 (1:500, ab182422, Abcam, Cambridge, MA, USA), PGP9.5 (1:1000, ab108986, Abcam, Cambridge, MA, USA), ChAT (1:2000, ab178850, Abcam, Cambridge, MA, USA), and TH (1:3000, ab137869, Abcam, Cambridge, MA, USA) at 4°C overnight. .. On the following day, tissues were incubated using biotinylated secondary antibody for 2 h. To visualize the antibody binding, a Article Title: Dietary Supplementation of Edible Mushroom Phallus atrovolvatus Aqueous Extract Attenuates Brain Changes in the App NL−G−F Mouse Model of Alzheimer’s Disease Article Snippet: After antigen retrieval, the brain sections were subsequently incubated in 0.3% H 2 O 2 for 5 min, then blocked in PBS containing 5% normal goat serum (Equitech-Bio, Kerrville, TX, USA), 0.5% bovine serum albumin (Equitech-Bio, Kerrville, TX, USA), 0.1% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA), and 0.02% sodium azide for at least 1 h. Afterward, the sections were incubated in the primary antibody at 4 °C overnight, washed, and incubated in a biotinylated secondary antibody (1:2000) for 2 h at room temperature. .. Next, the sections were further incubated using a Article Title: Dietary Supplementation of Edible Mushroom Phallus atrovolvatus Aqueous Extract Attenuates Brain Changes in the App NL-G-F Mouse Model of Alzheimer's Disease. Article Snippet: After antigen retrieval, the brain sections were subsequently incubated in 0.3% H2O2 for 5 min, then blocked in PBS containing 5% normal goat serum (Equitech-Bio, Kerrville, TX, USA), 0.5% bovine serum albumin (Equitech-Bio, Kerrville, TX, USA), 0.1% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA), and 0.02% sodium azide for at least 1 h. Afterward, the sections were incubated in the primary antibody at 4 ◦C overnight, washed, and incubated in a biotinylated secondary antibody (1:2000) for 2 h at room temperature. .. Next, the sections were further incubated using a Immunostaining:Article Title: Acute LPS exposure enhances susceptibility to peripheral prion infection. Article Snippet: Macrophages were detected in the spleen using F4/80 mAb (clone CI: A3-1; Bio-Rad Laboratories; 1/100 dilution). .. Following addition of biotinylated secondary antibodies, immunostaining was revealed using the |